The question was, 'Is there a way of minimizing the amount of damage you're doing so that you can then study cells in a physiological manner while also studying them at high spatial and temporal resolution for a long time?'
Sometimes I make an analogy that each scientific paper is like putting out another record. And some people have careers that are nothing but a one-hit wonder. And then there are people who are only appreciated by aficionados but largely forgotten by the wider community.
In my opinion, the only real asset one has is one's reputation, right? I mean, any company and institution can go belly up at any time. But if you have a good reputation, you know, you can usually find somebody who can - who thinks they can use what you have to offer.
I was born in 1960 and can still tell you the name of every astronaut from Mercury to Apollo. If I had a chance, I'd love to go into space on one of the privately developed space crafts.
Frankly, I guess, I don't really understand why people, why so many people, are so risk averse. You know, there's always ways to wiggle your way out of any situation if you're motivated enough.
It's nice to be able to look at one protein, but life is driven by the interactions between proteins, so it's really essential to be able to see multiple proteins at a time to understand these interactions.